Herbert West, Re-Animator, as played by Jeffrey Combs in the film of the same name (and Bride of Re-Animator, though mainly because we get insane stuff like Herb doodling with body-parts while his housemate/partner Dr Dan Cain has sex with a woman upstairs--it's like he can't quite figure out why he's so upset, but it keeps on resulting in spiders made out of fingers and eyeballs that scurry around the place until stepped on). Herb remains top of my list of formative Bossy Little Nutters with glasses, the kind of guy you can't leave with a dead body for more than two minutes without him pulling out the reagent and seeing just how good that residual brainstem activity is. (Never quite good enough, but he sure does keep on trying.)

Runner(s)-up: Sexy and unreliable sociopath with a scalpel Herr Baron-Doktor Victor Frankenstein, as played by Peter Cushing in the Hammer movies, and sexy and unreliable swashbuckling virologist Dr David Sandstrom, as played by Peter Outerbridge in the Canadian TV series ReGenesis.